HPLC SEPARATION GUIDE – Analysis of Nitroaromatic Explosives
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Introduction
Energetic materials such as explosives have become an environmental concern due to some of their toxic properties1,2. The need for disposal of aging military munitions and an increased concern about their distribution in the environment have become a priority for the US Environmental Protection Agency...
HPLC SEPARATION GUIDE – Standard C18 column in high throughput LC-MS applications
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Introduction
In today’s paradigm of drug discovery, high throughput screening of hit-to-lead compounds is commonplace. Researchers screen millions of chemical compounds found in commercially available and proprietary libraries in search of a lead molecule, which reacts in a predefined biochemical process…
HPLC SEPARATION GUIDE – Analysis of Fat-Soluble Vitamins
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Introduction
Vitamins are of unique importance to the development and function of nerve cells in that they participate in virtually all of the metabolic systems that provide energy and structural components for the nervous system. Vitamins can be grouped as fat-soluble and water-soluble…
HPLC SEPARATION GUIDE – Identification of Mycobacterium . Intracellulare by HPLC and Computer Driven Pattern Recognition System
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Introduction
The cell envelopes of all Mycobacteria contain high molecular weight -branched, B-hydroxy fatty acids components called mycolic acids. All known mycolic acids have the basic structure: where R1 is a C20 to C24 linear alkane and R2 is a more complex structure of 30 to 60 carbon atoms…
HPLC SEPARATION GUIDE – Separation of the Gentamicin Complex
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Introduction
The antibiotic gentamicin complex consists of at least four closely related isomeric pseudo-oligosaccharides referred to as gentamicin C1, C1a, C2 and C2a (see Figure 1). Gentamicin complex is produced by fermentation of Micromonospora purpurea…
HPLC SEPARATION – Analysis of Antibacterial Residues in Cow Milk
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Introduction
Flavonoids occur naturally in plant foods and are a common component of our diet. Flavonoids have demonstrated a wide range of biochemical and pharmacological effects, including anti-inflammatory and anti-allergic effects...
HPLC SEPARATION GUIDE – Analysis of Flavonoids in Green Tea
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Introduction
Flavonoids occur naturally in plant foods and are a common component of our diet. Flavonoids have demonstrated a wide range of biochemical and pharmacological effects, including anti-inflammatory and anti-allergic effects…
HPLC SEPARATION GUIDE – Analysis of Priority Pollutant: Polycyclic Aromatic Hydrocarbons
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Introduction
Polycyclic Aromatic Hydrocarbons (PAHs) are large organic compounds produced during combustion. Many of these compounds are carcinogenic and are often found in water, air (e.g. PAHs absorbed on airborne particulates) and other environmental locations…
HPLC SEPARATION GUIDE – Analysis of Active Ingredients in a Sun Screen
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Introduction
The main purpose of sun-screening preparations is to protect human skin from the damaging effects of light. By far the best-known light-induced skin damage is light erythema, sunburn. It is caused by ultraviolet B (280-315nm) rays…
HPLC SEPARATION GUIDE – Separation of Nucleotides
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Introduction
In every living cell there are found nucleoproteins: substances made up of proteins combined with natural polymer of another kind, the nucleic acids. The backbone of the nucleic acid molecule consists of a polyester chain (called a polynucleotide chain)…