Bulk Packings


Sequencing Grade Tandem Mass Spectrometry for Top−Down Proteomics Using Hybrid Electron Capture Dissociation Methods in a Benchtop Orbitrap Mass Spectrometer — SMT REFERENCED ARTICLE

Shaw JB, Malhan N, Vasil’ev YV, Lopez NI, Makarov A, Beckman JS, Voinov VG
Analytical Chemistry, 2018

American Journal of Microbiological Research, Separation Methods TechnologiesCompared to traditional collision induced dissociation methods, electron capture dissociation (ECD) provides more comprehensive characterization of large peptides and proteins as well as preserves labile post-translational modifications. However, ECD experiments are generally restricted to the high magnetic fields of FTICR-MS that enable the reaction of large polycations and electrons. Here, we demonstrate the use of an electromagnetostatic ECD cell to perform ECD and hybrid ECD methods utilizing 193 nm photons (ECuvPD) or collisional activation (EChcD) in a benchtop quadrupole-Orbitrap mass spectrometer…and were packed in-house with 3 μm 300 Å C2 [BMEB2-5-300] reversed phase resin (Separation Methods Technologies, INC., Newark, DE). mAb IdeS digest (0.1 μg) was loaded onto the trap column and washed for 10 min at 5 μL/min with 1% mobile phase B…READ MORE HERE


SMT SAM Reversed-Phase C18 Column

SMT SAM C18 HPLC Column and Chromatography Media

• High density octadecyl (C18) ligand, achieved through meticulous mixture of the C18 with proprietary spacer molecules to ensure maximum coverage

• Very stable at a wide pH range (1-12) and high temperatures

• Extremely sensitive and exceptional performance

• Carbon Load – OD Series: ~24%; Elite C18 Series: ~16%; ODL Series: ~12%
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SMT SAM WCX-O Mix-Mode Column

SMT SAM WCX-O Mixed-Mode silica-based columns are manufactured with weak cation exchange and hydrophobic alkyl functional groups. These advanced columns are ideal for reversed-phase, weak cation-exchange, and hydrophilic interaction liquid chromatography modes. In addition, these columns provide superior selectivity and efficiency in the separation of proteins and other biomolecules.
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Informed-Proteomics: open-source software package for top-down proteomics — SMT REFERENCED ARTICLE

Jungkap Park, Paul D Piehowski, Christopher Wilkins, Mowei Zhou, Joshua Mendoza, Grant M Fujimoto, Bryson C Gibbons, Jared B Shaw, Yufeng Shen, Anil K Shukla, Ronald J Moore, Tao Liu, Vladislav A Petyuk, Nikola Tolić, Ljiljana Paša-Tolić, Richard D Smith, Samuel H Payne & Sangtae Kim
Nature Methods, Volume 14

Nature Methods, Volume 14 Separation Methods TechnologiesTop-down proteomics, the analysis of intact proteins in their endogenous form, preserves valuable information about post-translation modifications, isoforms and proteolytic processing. The quality of top-down liquid chromatography–tandem MSMS (LCLC-MSMS/MSMS) data sets is rapidly increasing on account of advances in instrumentation and sample-processing protocols. However, top-down mass spectra are substantially more complex than conventional bottom-up data…The analytical column was prepared in house by slurry packing 3 μm diameter C2 [BMEB C2] stationary phase (Separation Methods Technology, Newark, Delaware) into a 50 cm length of 360 μm o.d. × 100 μm i.d. fused silica capillary column…READ MORE HERE


SMT MetalSep Resin

SMT MetalSep Resin is offered as bulk packing materials for low pressure liquid chromatography, solid phase extraction, preparatory and process scale applications. The packings consist of proprietary strong cation exchange functional ligands that are chemically attached on silica substrate using Total Coverage technology. Total Coverage technology utilizes advanced Self-Assembled Monolayers (SAM) to achieve bonded phase coverages that approach 100%. Our method of bonding allows the density of the functional ligands to be controlled with appropriate spacer molecules, a procedure that ensures highly cross-linked polysiloxane under layer structure. The results are bonded phases that are well protected and that show unprecedented resistance to both acid and base hydrolysis. The packing materials offer strong selectivities toward heavy metal ions such as Copper, gold, nickel, silver, iron, etc.
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