Bulk Packings

30May/18

Informed-Proteomics: open-source software package for top-down proteomics — SMT REFERENCED ARTICLE

Jungkap Park, Paul D Piehowski, Christopher Wilkins, Mowei Zhou, Joshua Mendoza, Grant M Fujimoto, Bryson C Gibbons, Jared B Shaw, Yufeng Shen, Anil K Shukla, Ronald J Moore, Tao Liu, Vladislav A Petyuk, Nikola Tolić, Ljiljana Paša-Tolić, Richard D Smith, Samuel H Payne & Sangtae Kim
Nature Methods, Volume 14

Nature Methods, Volume 14 Separation Methods TechnologiesTop-down proteomics, the analysis of intact proteins in their endogenous form, preserves valuable information about post-translation modifications, isoforms and proteolytic processing. The quality of top-down liquid chromatography–tandem MSMS (LCLC-MSMS/MSMS) data sets is rapidly increasing on account of advances in instrumentation and sample-processing protocols. However, top-down mass spectra are substantially more complex than conventional bottom-up data…The analytical column was prepared in house by slurry packing 3 μm diameter C2 [BMEB C2] stationary phase (Separation Methods Technology, Newark, Delaware) into a 50 cm length of 360 μm o.d. × 100 μm i.d. fused silica capillary column…READ MORE HERE

07Apr/17

SMT MetalSep Resin


SMT MetalSep Resin is offered as bulk packing materials for low pressure liquid chromatography, solid phase extraction, preparatory and process scale applications. The packings consist of proprietary strong cation exchange functional ligands that are chemically attached on silica substrate using Total Coverage technology. Total Coverage technology utilizes advanced Self-Assembled Monolayers (SAM) to achieve bonded phase coverages that approach 100%. Our method of bonding allows the density of the functional ligands to be controlled with appropriate spacer molecules, a procedure that ensures highly cross-linked polysiloxane under layer structure. The results are bonded phases that are well protected and that show unprecedented resistance to both acid and base hydrolysis. The packing materials offer strong selectivities toward heavy metal ions such as Copper, gold, nickel, silver, iron, etc.
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24Jan/17

High-Resolution Ultrahigh-Pressure Long Column Reversed-Phase Liquid Chromatography for Top-down Proteomics — SMT REFERENCED ARTICLE

Yufeng Shen, Nikola Tolić, Paul D. Piehowski, Anil K. Shukla, Sangtae Kim, Rui Zhao, Yi Qu, Errol Robinson, Richard D. Smith
Journal of Chromatography A

Journal-of-Chromatography-A Separation Methods TechnologiesSeparation of proteoforms for global intact protein analysis (i.e. top-down proteomics) has lagged well behind what is achievable for peptides in traditional bottom-up proteomic approach and is becoming a true bottle neck for top-down proteomics. Herein, we report use of long (≥1 M) columns containing short alkyl (C1-C4) bonded phases to achieve high-resolution RPLC for separation of proteoforms…The packing…silica particles, were from…Separation Methods Technologies (Newark, DE) [3µm, 300Å C1 porous silica (BMEB1-3-300) and 3µm, 300Å C2 porous silica (BMEB2-3-300)]…C1 RPLC has the greatest potential in terms of elution of hydrophobic and larger proteoforms; accordingly, we observed dense peak arrays in the C1 RPLC late elution zone…READ MORE HERE

31May/16

Novel Biosensor of Membrane Protein Proximity Based on Fluorogen Activated Proteins — SMT REFERENCED ARTICLE

V. Vasilev, Kalin; Gallo, Eugenio; Shank, Nathaniel; W. Jarvik, Jonathan
Combinatorial Chemistry & High Throughput Screening

CCTHSThe biosensor takes advantage of recently developed fluorogen-activating proteins (FAPs) that display fluorescence only when bound to otherwise-nonfluorescent fluorogen molecules…The bright blue product was purified…with a column packed with Bulk C18 (Separation Methods Technologies) [BOD-35-150] using a linear gradient of 0.1% trifluoroacetic acid (TFA) in H2O and acetonitrile (ACN) (0% to 40% ACN over 40…READ MORE HERE

06Apr/16

Visit SMT at PREP Symposium 2016 in Philadelphia, PA – Booth #102

prep-2016
Visit Us at the PREP 2016: 29th International Symposium on Preparative and Process Chromatography
Most recognized international conference in the world for the presentation and discussion of the latest developments driving the field of Preparative & Process Chromatography, Separation Science and Technology
Location: The Loews Hotel – Philadelphia, PA USA – Booth #102
Dates: July 17-20, 2016