Columns

21Aug/18

Extraction of Phytochemicals from Eucalyptus Spp. & Withania Somnifera and Their Biological Testing — SMT REFERENCED ARTICLE

Praveen Kumar Gupta, Priyanka V, Lingayya Hiremath, S Narendra Kumar, Ajeet Kumar Srivastava
American Journal of Microbiological Research, 2018

American Journal of Microbiological Research, Separation Methods TechnologiesDisease incidence and prevalence is increasing in developing countries leading to high mortality and morbidity rates. Since most of the developing countries rely on traditional plant based medicine, there is huge demand for identifying the bioactive compounds from plant origin with medicinal properties owing to their safety, availability and reduced side effects. This project focuses on extraction of two different phytochemicals from Eucalyptus Spp and Withania Somnifera…HPLC analysis was carried out on a LC-20AT system (Shimadzu, Japan) using a reverse-phase C18 column [OD-5-100/25] (4.6 mm x 250 mm; Separation Methods Technologies)…READ MORE HERE

30Jul/18

SMT SAM Reversed-Phase C18 Column

SMT SAM C18 HPLC Column and Chromatography Media

• High density octadecyl (C18) ligand, achieved through meticulous mixture of the C18 with proprietary spacer molecules to ensure maximum coverage

• Very stable at a wide pH range (1-12) and high temperatures

• Extremely sensitive and exceptional performance

• Carbon Load – OD Series: ~24%; Elite C18 Series: ~16%; ODL Series: ~12%
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17Jul/18

SMT SAM WCX-O Mix-Mode Column


SMT SAM WCX-O Mixed-Mode silica-based columns are manufactured with weak cation exchange and hydrophobic alkyl functional groups. These advanced columns are ideal for reversed-phase, weak cation-exchange, and hydrophilic interaction liquid chromatography modes. In addition, these columns provide superior selectivity and efficiency in the separation of proteins and other biomolecules.
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30May/18

Informed-Proteomics: open-source software package for top-down proteomics — SMT REFERENCED ARTICLE

Jungkap Park, Paul D Piehowski, Christopher Wilkins, Mowei Zhou, Joshua Mendoza, Grant M Fujimoto, Bryson C Gibbons, Jared B Shaw, Yufeng Shen, Anil K Shukla, Ronald J Moore, Tao Liu, Vladislav A Petyuk, Nikola Tolić, Ljiljana Paša-Tolić, Richard D Smith, Samuel H Payne & Sangtae Kim
Nature Methods, Volume 14

Nature Methods, Volume 14 Separation Methods TechnologiesTop-down proteomics, the analysis of intact proteins in their endogenous form, preserves valuable information about post-translation modifications, isoforms and proteolytic processing. The quality of top-down liquid chromatography–tandem MSMS (LCLC-MSMS/MSMS) data sets is rapidly increasing on account of advances in instrumentation and sample-processing protocols. However, top-down mass spectra are substantially more complex than conventional bottom-up data…The analytical column was prepared in house by slurry packing 3 μm diameter C2 [BMEB C2] stationary phase (Separation Methods Technology, Newark, Delaware) into a 50 cm length of 360 μm o.d. × 100 μm i.d. fused silica capillary column…READ MORE HERE