Jungkap Park, Paul D Piehowski, Christopher Wilkins, Mowei Zhou, Joshua Mendoza, Grant M Fujimoto, Bryson C Gibbons, Jared B Shaw, Yufeng Shen, Anil K Shukla, Ronald J Moore, Tao Liu, Vladislav A Petyuk, Nikola Tolić, Ljiljana Paša-Tolić, Richard D Smith, Samuel H Payne & Sangtae Kim
Nature Methods, Volume 14
Top-down proteomics, the analysis of intact proteins in their endogenous form, preserves valuable information about post-translation modifications, isoforms and proteolytic processing. The quality of top-down liquid chromatography–tandem MSMS (LCLC-MSMS/MSMS) data sets is rapidly increasing on account of advances in instrumentation and sample-processing protocols. However, top-down mass spectra are substantially more complex than conventional bottom-up data…The analytical column was prepared in house by slurry packing 3 µm diameter C2 [BMEB C2] stationary phase (Separation Methods Technology, Newark, Delaware) into a 50 cm length of 360 µm o.d. × 100 µm i.d. fused silica capillary column…READ MORE HERE
