Novel Biosensor of Membrane Protein Proximity Based on Fluorogen Activated Proteins – SMT REFERENCED ARTICLE

V. Vasilev, Kalin; Gallo, Eugenio; Shank, Nathaniel; W. Jarvik, Jonathan
Combinatorial Chemistry & High Throughput Screening

CCTHSThe biosensor takes advantage of recently developed fluorogen-activating proteins (FAPs) that display fluorescence only when bound to otherwise-nonfluorescent fluorogen molecules…The bright blue product was purified…with a column packed with Bulk C18 (Separation Methods Technologies) [BOD-35-150] using a linear gradient of 0.1% trifluoroacetic acid (TFA) in H2O and acetonitrile (ACN) (0% to 40% ACN over 40…READ MORE HERE