Sequencing Grade Tandem Mass Spectrometry for Top-Down Proteomics Using Hybrid Electron Capture Dissociation Methods in a Benchtop Orbitrap Mass Spectrometer – SMT REFERENCED ARTICLE

Shaw JB, Malhan N, Vasil’ev YV, Lopez NI, Makarov A, Beckman JS, Voinov VG
Analytical Chemistry, 2018

American Journal of Microbiological Research, Separation Methods TechnologiesCompared to traditional collision induced dissociation methods, electron capture dissociation (ECD) provides more comprehensive characterization of large peptides and proteins as well as preserves labile post-translational modifications. However, ECD experiments are generally restricted to the high magnetic fields of FTICR-MS that enable the reaction of large polycations and electrons. Here, we demonstrate the use of an electromagnetostatic ECD cell to perform ECD and hybrid ECD methods utilizing 193 nm photons (ECuvPD) or collisional activation (EChcD) in a benchtop quadrupole-Orbitrap mass spectrometer…and were packed in-house with 3 µm 300 AÌŠ C2 [BMEB2-5-300] reversed phase resin (Separation Methods Technologies, INC., Newark, DE). mAb IdeS digest (0.1 μg) was loaded onto the trap column and washed for 10 min at 5 µL/min with 1% mobile phase B…READ MORE HERE