Shaw JB, Malhan N, Vasil’ev YV, Lopez NI, Makarov A, Beckman JS, Voinov VG
Analytical Chemistry, 2018
Compared to traditional collision induced dissociation methods, electron capture dissociation (ECD) provides more comprehensive characterization of large peptides and proteins as well as preserves labile post-translational modifications. However, ECD experiments are generally restricted to the high magnetic fields of FTICR-MS that enable the reaction of large polycations and electrons. Here, we demonstrate the use of an electromagnetostatic ECD cell to perform ECD and hybrid ECD methods utilizing 193 nm photons (ECuvPD) or collisional activation (EChcD) in a benchtop quadrupole-Orbitrap mass spectrometer…and were packed in-house with 3 µm 300 AÌŠ C2 [BMEB2-5-300] reversed phase resin (Separation Methods Technologies, INC., Newark, DE). mAb IdeS digest (0.1 μg) was loaded onto the trap column and washed for 10 min at 5 µL/min with 1% mobile phase B…READ MORE HERE
