Development and Validation of HPLC Method for Simultaneous Estimation of Ketorolac Tromethamine and Ofloxacin in Bulk and Ophthalmic Formulation — SMT REFERENCED ARTICLE

Tambe Vrushali Sachin, Dr. Deodhar Meenakshi, Dr. Prakya Vijayalakshmi
Indo American Journal of Pharmaceutical Research, 2015

A novel validated HPLC method was developed for simultaneous determination of Ofloxacin and Ketorolac Tromethamine from bulk and eye drop formulation. This method is simple, precise, accurate and utilizes reverse phase SMT SAM-C18 column (4.6 × 250 mm) with a ternary mobile phase with pH adjustment to 2.7 with formic acid, at a flow rate of 1ml/min. Both drugs were qunatitated at 300 nm. The retention time for OFO and KTM was found to be 2.33 and 10.33 min respectively…