Reversed-Phase MEB4 C4 Guard Column
Guard columns are expendable small columns designed to remove anything that may interfere with the separation or shorten the lifetime of the primary column. A standard HPLC column (analytical or preparative) is hereby referred to as the primary because it is first in value and in importance for separation.
Guard columns are utilized to remove:
- Particles that may clog the primary column and necessitate a frit change, which can be cumbersome.
- Compounds, including ions, that may adsorb strongly on the packing material and cause baseline drift, spurious peaks, loss of resolution and change in selectivity
- Compounds that can form a precipitate upon contact with mobile phase or packing
- Compounds that may co-elute with analyte and interfere with its detection and quantitation.
SMT guard is designed to have minimal or no effect on separation. The concept is verified by injection of standards before and after guard installation. The small increase in retention is due to the added packing materials in the guard device. The column performance and peak symmetry are not altered in the separation because SMT guard is packed with the same pressure as the primary column and has minimal void volume design as shown in the chromatogram. Because of the way it is packed, the guard can also be used alone as an analytical column.
Are you looking for a column with custom specifications (pore size, particle size, length and/or I.D.) not available on this site? Please contact us as we can manufacture the exact column you need.