Chromatography Consumables & Columns Overview

 

Separation Methods Technologies, Inc. (SMT) has built a reputation as a manufacturer of the highest quality chromatography consumables & columns. We offer a wide selection of silica sizes of various pore dimensions, for various chromatographic applications. The Horizontal Polymerized Bonded Phase packing material offers the benefits of maximized silica life, which reduces manufacturing down time, waste disposal, and increased overall efficiency.

 

HPLC Columns pH Effects Comparison

Reversed Phase Columns

The use of high performance liquid chromatography (HPLC) for the separation and purification of organic compounds including pharmaceuticals, natural products, food additives, organic chemicals and biologicals, has increased dramatically in the past three decades. This increase is undoubtedly associated with the enormous improvement in bonding chemistry.

Various types of Reversed Phase Columns SMT has to offer:

Methyl (C1)
Ethyl (C2)

Propyl (C3)
Butyl (C4)
Pentyl (C5)
Hexyl (C6)
Octyl (C8)
Octadecyl (C18)
Phenyl-Hexyl

 

Normal Phase Columns

When chromatographic separation is done in a normal phase mode, the surface chemistry of the stationary phase has a polar characteristic. The mobile phase is generally nonpolar organic solvent (such as hexane or heptane).

Normal Phase Columns SMT has to offer:

Silica
Diol
Aminopropyl (NH2)
Cyanopropyl (CN)

 

Ion Exchange Columns

SMT has developed new series of ion-exchange packing materials with its novel SAM technology. These chromatography consumables & columns are offered for all stages of separation science from analytical scale levels to process scale purification levels. The packings are generally silica-based and consist of totally porous particles with approximately 1 Meq/g capacity. SMT packing materials allow flexibility of mobile phases.

Ion Exchange Columns SMT has to offer:

Strong Anion eXchange (SAX)
Weak Anion eXchange (WAX)
Strong Cation eXchange (SCX)

Weak Cation eXchange (WCX)
Diethylaminoethyl (DEAE)

SMT C18 Columns vs Waters and Agilent and Phenomenex

Various types of Reversed Phase Columns SMT has to offer:

Methyl (C1)
Ethyl (C2)

Propyl (C3)
Butyl (C4)
Pentyl (C5)
Hexyl (C6)
Octyl (C8)
Octadecyl (C18)
Phenyl-Hexyl

 

Normal Phase Columns

When chromatographic separation is done in a normal phase mode, the surface chemistry of the stationary phase has a polar characteristic. The mobile phase is generally nonpolar organic solvent (such as hexane or heptane).

Normal Phase Columns SMT has to offer:

Silica
Diol
Aminopropyl (NH2)
Cyanopropyl (CN)

 

Ion Exchange Columns

SMT has developed new series of ion-exchange packing materials with its novel SAM technology. These chromatography consumables & columns are offered for all stages of separation science from analytical scale levels to process scale purification levels. The packings are generally silica-based and consist of totally porous particles with approximately 1 Meq/g capacity. SMT packing materials allow flexibility of mobile phases.

Ion Exchange Columns SMT has to offer:

Strong Anion eXchange (SAX)
Weak Anion eXchange (WAX)
Strong Cation eXchange (SCX)

Weak Cation eXchange (WCX)
Diethylaminoethyl (DEAE)

Specialty Columns

We have a special interest in surface modification and materials engineering. When separation is difficult with conventional bonded phases, SMT assists in method development and specialty chromatography column design for new applications. SMT specialty columns include special columns designed for reversed phase, normal phase, and ion exchange chromatography. These columns are specially designed for companies that are interested in having competitive advantage in separation and surface modification.

Specialty Columns SMT has to offer:

PAH
Analysis of Polyaromatic Hydrocarbons
TNT
Separation of Explosives
AquSep1 (OD-AQ) & AquSep2
Polar/Nonpolar/Basic Compounds
C12
Nonpolar/Polar Compounds
C30
Nonpolar Compounds
Urea
Polar Compounds
QuickSep
Quick Screening/Analysis
ChiralSep
Enantiomers
MetalSep
Metal Removal
C6F5
Separation of Taxols
CIB Ovomucoid, Teicoplanin, ProteinA & IgG
Chiral Separation
USP
Regulated Analytical Methods
Micro/Narrow Bore
LC/MS, LC/GC, Drug Screening
Guard
Column Guard

Bulk Sorbents and Packing Material

SMT Total Coverage™ chromatography packings are designed for usage from analytical to process scale purification. The primary purpose for analytical method developments in pharmaceutical and food industries is for subsequent process scale purification. The analytical steps are used to reduce the cost incurable in erroneous large-scale purification steps. SMT is a specialty column company, and as such, offers services that can greatly enhance scale-up of many analytical methods development. Bulk samples are offered for your initial evaluation. SMT offers a variety of packing materials in bulk quantities for reversed-phase, normal phase, and ion exchange chromatography. These B series bulk packing materials usually consist of silica substrates and are made with Total Coverage™ technology and guaranteed to last much longer than competitor’s silica-based packings. Polymer-based packings are made available for special applications that require use in extreme pH conditions (such as pH=14).

Bulk Packings SMT has to offer:

Bulk Silica
Bulk C1
Bulk C2
Bulk C4
Bulk C8
Bulk C18
Bulk SAX
Bulk SCX
Bulk DEAE
Bulk Diol (OH)
Bulk Aminopropyl AP (NH2)
Bulk Cyanopropyl CP (CN)
Bulk Phenyl
Bulk Phenyl-Hexyl
Bulk WAX
Bulk WCX
Bulk MetalSep
And other Custom Packings