- Extraction of Phytochemicals from Eucalyptus Spp. & Withania Somnifera and Their Biological Testing: June 2018
Praveen Kumar Gupta, Priyanka V, Lingayya Hiremath, S Narendra Kumar, Ajeet Kumar Srivastava
American Journal of Microbiological Research, 2018, Vol. 6, No. 4, 115-123
Disease incidence and prevalence is increasing in developing countries leading to high mortality and morbidity rates. Since most of the developing countries rely on traditional plant based medicine, there is huge demand for identifying the bioactive compounds from plant origin with medicinal properties owing to their safety, availability and reduced side effects. This project focuses on extraction of two different phytochemicals from Eucalyptus Spp and Withania Somnifera…HPLC analysis was carried out on a LC-20AT system (Shimadzu, Japan) using a reverse-phase C18 column [OD-5-100/25] (4.6 mm x 250 mm; Separation Methods Technologies)… - Informed-Proteomics: open-source software package for top-down proteomics: August 2017
Jungkap Park, Paul D Piehowski, Christopher Wilkins, Mowei Zhou, Joshua Mendoza, Grant M Fujimoto, Bryson C Gibbons, Jared B Shaw, Yufeng Shen, Anil K Shukla, Ronald J Moore, Tao Liu, Vladislav A Petyuk, Nikola Tolić, Ljiljana Paša-Tolić, Richard D Smith, Samuel H Payne & Sangtae Kim
Nature Methods, Volume 14, pg 909–914
Top-down proteomics, the analysis of intact proteins in their endogenous form, preserves valuable information about post-translation modifications, isoforms and proteolytic processing. The quality of top-down liquid chromatography–tandem MSMS (LCLC-MSMS/MSMS) data sets is rapidly increasing on account of advances in instrumentation and sample-processing protocols. However, top-down mass spectra are substantially more complex than conventional bottom-up data… The analytical column was prepared in house by slurry packing 3 µm diameter C2 stationary phase (Separation Methods Technology, Newark, Delaware) into a 50 cm length of 360 µm o.d. × 100 µm i.d. fused silica capillary column… - High-Resolution Ultrahigh-Pressure Long Column Reversed-Phase Liquid Chromatography for Top-down Proteomics: May 2017
Yufeng Shen, Nikola Tolić, Paul D. Piehowski, Anil K. Shukla, Sangtae Kim, Rui Zhao, Yi Qu, Errol Robinson, Richard D. Smith
Journal of Chromatography A. 2017 pii: S0021-9673(17)30022-5
Separation of proteoforms for global intact protein analysis (i.e. top-down proteomics) has lagged well behind what is achievable for peptides in traditional bottom-up proteomic approach and is becoming a true bottle neck for top-down proteomics. Herein, we report use of long (≥1 M) columns containing short alkyl (C1-C4) bonded phases to achieve high-resolution RPLC for separation of proteoforms…The packing…silica particles, were from…Separation Methods Technologies (Newark, DE) [3µm, 300Å C1 porous silica (BMEB1-3-300) and 3µm, 300Å C2 porous silica (BMEB2-3-300)]…C1 RPLC has the greatest potential in terms of elution of hydrophobic and larger proteoforms; accordingly, we observed dense peak arrays in the C1 RPLC late elution zone… - New Chromatography Columns and Accessories for 2017: April 2017
Dave S.Bell
LCGC North America Volume 35, Issue 4, pg 226–239
Separation Methods Technologies commercialized an interesting set of short-chain reversed-phase chemistries (C1, C2, and C4). The company contends that their proprietary bonding strategy provides “proper deactivation†of silanols as well as resistance to acid hydrolysis that plagues many short-chain surface chemistries… Separation Methods Technologies reported the development of a teicoplanin-based chiral stationary phase. The company reports that the column is useful for performing a wide variety of chiral separations with amino acids, carboxylic acids and neutral compounds… - Novel Biosensor of Membrane Protein Proximity Based on Fluorogen Activated Proteins
V. Vasilev, Kalin; Gallo, Eugenio; Shank, Nathaniel; W. Jarvik, Jonathan
Combinatorial Chemistry & High Throughput Screening, Volume 19, Number 5, June 2016, pp. 392-399(8)
The biosensor takes advantage of recently developed fluorogen-activating proteins (FAPs) that display fluorescence only when bound to otherwise-nonfluorescent fluorogen molecules…The bright blue product was purified…with a column packed with Bulk C18 (Separation Methods Technologies) using a linear gradient of 0.1% trifluoroacetic acid (TFA) in H2O and acetonitrile (ACN) (0% to 40% ACN over 40… - New Chromatography Columns and Accessories for 2016: April 2016
Dave Bell
LCGC North America Volume 34, Issue 4, pg 242–252
Separation Methods Technologies introduced an FPP version of a C30 chemistry… Separation Methods Technologies launched a protein A column based on FPP substrate. According to the company, a proprietary bonding strategy using self-assembled monolayers (SAMs) ensures complete removal of nonspecific binding sites. Protein A has been shown to provide high binding capacity for mammalian IgG… - Interactions of Haptoglobin with Monomeric Globin Species: Insights from Molecular Modeling and Native Electrospray Ionization Mass Spectrometry: March 2016
Ololade Fatunmbi, Rinat R. Abzalimov, Sergey N. Savinov, Anne Gershenson & Igor A. Kaltashov
Biochemistry, 2016
Haptoglobin (Hp) binds free hemoglobin (Hb) dimers to prevent negative consequences of Hb circulation in the extracellular environment. Although both monomeric Hb and myoglobin (Mb) species also present potential risks, their interactions with Hp have not been extensively studied. Mb is homologous to both the α- and β-chains of Hb and shares many conserved Hb/Hp interface residues, yet whether Hp binds Mb remains unclear…Hb α- and β-chains were separated via reversed phase high-performance liquid chromatography…using a 4.6 mm × 150mm C8 [O-5-100/15] analytical column with a 5 µm pore size (Separation Methods Technologies, Newark, DE)… - Kinetic and mechanistic studies of the reactivity of iron(IV) TAMLs toward organic sulfides in water: resolving a fast catalysis versus slower single-turnover reactivity dilemma: July 2015
Deboshri Banerjee, Alexander D. Ryabov & Terrence J. Collins
Journal of Coordination Chemistry, 2015
TAML complex is oxidized by H2O2 or tBuOOH in water at pH < 10 into the corresponding iron(IV) μ-oxo-bridged dimer 2, which oxidizes readily ring-substituted thioanisoles p-XC6H4SMe (X=H, MeO, Me, Cl, CN) into the corresponding sulfoxides with regeneration of 1. The oxidation studied under pseudo-first-order conditions using the stopped-flow technique by monitoring the fading of the 420-nm band of 2 follows hyperbolic kinetics according to the rate law kobs = ab[p-XC6H4SMe]/(1 + b[p-XC6H4SMe]) at pH 8 and 25 °C…. Orange II was purified by passing through a column filled with SMT Bulk-C18 (Separation Methods Technologies Inc., Newark) with water–methanol mixture (9 : 1) as an eluent… - Development and Validation of HPLC Method for Simultaneous Estimation of Ketorolac Tromethamine and Ofloxacin in Bulk and Opthalmic Formulation: February 2015
Tambe Vrushali Sachin, Dr. Deodhar Meenakshi, Dr. Prakya Vijayalakshmi
Indo American Journal of Pharmaceutical Research, 2015
A novel validated HPLC method was developed for simultaneous determination of Ofloxacin and Ketorolac Tromethamine from bulk and eye drop formulation. This method is simple, precise, accurate and utilizes reverse phase SMT SAM-C18 column (4.6×250 mm) with a ternary mobile phase with pH adjustment to 2.7 with formic acid, at a flow rate of 1ml/min… - Immuno-proteomics: Development of a novel reagent for separating antibodies from their target proteins: October 2014
Vinitha Ganesan, Brigitte Schmidt, Raghunandan Avula, Dagney Cooke, Taylor Maggiacomo, Lawton Tellin, Dana P. Ascherman, Marcel P. Bruchez, Jonathan Minden
Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics
Immunoprecipitation (IP) is a widely used technique for identifying the binding partners of the target proteins of specific antibodies. Putative binding targets and their partners are usually in much lower amounts than the antibodies used to capture these target proteins. Thus antigen identification using proteomics following IP is often confounded by the presence of an overwhelming amount of interfering antibody protein….MPLC chromatography was performed on a Buchi Sepacore system using RP-18 (SMT Bulk-C18) manufactured by Separation Methods Technologies… - Recent Progress in Chiral Stationary Phase Development and Current Chiral Applications: April 2014
Timothy J. Ward, Karen D. Ward – LCGC Special Issues
…and an immobilized ovomucoid phase, C18-Ovo-5-120 from Separation Methods Technologies. Companies such as…Separation Methods Technologies are continuing to expand their offerings of immobilized polysaccharide-derived CSPs because these columns offer greater stability, can be used with a wider variety of mobile phases, and are useful in both liquid chromatography (LC) and SFC applications… - Characterization of the Structural Morphology of Chemically Modified Silica Prepared by Surface Polymerization of a Mixture of Long and Short Alkyl Chains Using 13C and 29Si NMR Spectroscopy: 2013, 29 (16), pp 4974–4987
Hafeez Fatunmbi and Martha D. Bruch
Langmuir
A series of bonded phases were prepared by the chemical modification of silica using the surface polymerization of trifunctional and difunctional ligands, and the structural morphology was characterized by solid-state nuclear magnetic resonance (NMR) spectroscopy using cross-polarization and magic angle spinning (CP/MAS). Mixed-phase surfaces were prepared using mixtures of trifunctional long-chain (C18) ligands with trifunctional…All samples were prepared using silica (Separation Methods Technologies, Inc., SMT-silica, catalog no. BS-5-60) with a pore size of 60 Å, a particle size of 5 µm, and a surface area of 500 m2/ g. BS-5-60 silica is very pure… - Spectrophotometric And Chromatographic Determination Of Acetylsalicylic Acid And Caffeine In Pure And In Tablet Dosage Form: 2012, 3(1): 73-81
Sonali S. Bharate, Sandip B. Bharate
In the present research paper, spectrophotometric and chromatographic assay method has been developed for the simultaneous estimation of acetylsalicylic acid and caffeine in bulk and commercial tablets. Acetylsalicylic acid and caffeine showed absorbance maxima at 228 and 275 nm in 0.1N HCl and at 225 and 272 nm in methanol, respectively. These drugs were estimated in formulations by simultaneous equation method (method A) and absorbance…these two components employed a SMT-C18, OD-5 100/25 (250×4.6 mm) column with a mobile phase of methanol using a UV detector… - Designing Green Oxidation Catalysts for Purifying Environmental Waters: June 2010
W. Chadwick Ellis, Camly T. Tran, Riddhi Roy, Marte Rusten, Andreas Fischer, Alexander D. Ryabov, Bruce Blumberg and Terrence J. Collins
We describe the synthesis, characterization, aqueous behavior, and catalytic activity of a new generation of FeIII-TAML (tetraamido macrocycle ligand) activators of peroxides (2), variants of [Fe{(OC)2(o,o′-NC6H4NCO)2CMe2}(OH2)-] (2d), which have been designed to be especially suitable for purifying water of recalcitrant oxidizable pollutants. Activation of H2O2 by 2 (kI) as a function of pH was analyzed via kinetic studies of Orange II bleaching. This was compared with the known behavior of the first generation of FeIII-TAMLs…Orange II was purified by column chromatography on reversed-phase silica gel using SMT-Bulk C18 no. BOD-35-150 resin (Separation Methods Technologies) using water as an eluent… - Combined supercritical fluid chromatographic tests to improve the classification of numerous stationary phases used in reversed-phase liquid chromatography: December 2008
West C, Fougère L, Lesellier E.
In this paper, we present a combination of a key-solute test based on retention and separation factors of large probe solutes (carotenoid pigments) and a quantitative structure-retention relationship analysis based on the retention factors of small probe solutes (aromatic compounds), both performed in supercritical fluid chromatography, to investigate the different chromatographic…SMT C18, 340 Specific surface area (m2 g-1), Self-assembled monolayer (SAM)… - Nucleoside reverse transcriptase inhibitors and their phosphorylated metabolites in human immunodeficiency virus-infected human matrices: June 2008
Jiaping Lai, Jun Wang, Zongwei Cai, J. Chromatogr B
Highly active antiretroviral therapy (HAART) is the common treatment strategy for human immunodeficiency virus (HIV)-infected patients at present. Generally, HAART regimens apply multi-therapy drugs that contain nucleoside/nucleotide reverse transcriptase inhibitors (NRTIs), non-nucleoside/nucleotide reverse transcriptase inhibitors (NNRTIs) and protease inhibitors (PIs). Unlike NNRTIs and PIs, the active form of NRTIs is not the drug itself but its triphosphorylated (TP) metabolites in intracellular medium…SMT-C18, 150 mm × 2.1 mm i.d… - FeIII – TAML-catalyzed green oxidative degradation of the azo dye Orange II by H2O2 and organic peroxides: products, toxicity, kinetics, and mechanisms: Green Chemistry 2007, 9, 49-57.
Naima Chahbane, Delia-Laura Popescu, Douglas A. Mitchell, Arani Chanda, Dieter Lenoir, Alexander D. Ryabov, Karl-Werner Schramma and Terrence J. Collins
Oxidation of Orange II ([4-[(2-hydroxynaphtyl)azo]benzenesulfonic acid], sodium salt) by hydrogen peroxide catalyzed by iron(III) complexed to tetra amido macrocyclic ligands (FeIII–TAML activators) in aqueous solutions at pH 9–11 leads to CO2, CO, phthalic acid and smaller aliphatic carboxylic acids as major mineralization products…Orange II was purified by passing through a column filled with SMT Bulk-C18 (Separation Methods Technologies Inc., Newark) - RP-HPLC determination of related substances Balofloxacin Study: Pharmacy November 2006
Objective RP-HPLC determination of related substances Balofloxacin. Method uses SMT C18 column (250mm × 4.6mm, 5µm), a mobile phase of acetonitrile -0.4% sodium dodecyl sulfate solution (47:53) [with citric acid (1.5mol / L) adjusted pH value to 3.0 ], detection wavelength 295nm, flow rate 1.0ml/min. Results Balofloxacin with all relevant material in the above-mentioned chromatographic conditions can be effectively separated. Conclusion The method is accurate… - Activity-Stability Parameterization of Homogeneous Green Oxidation Catalysts: Chemistry – A European Journal 2006, 12, 9336-9345.
Chanda A, Ryabov AD, Mondal S, Alexandrova L, Ghosh A, Hangun-Balkir Y, Horwitz CP, Collins TJ.
Small-molecule synthetic homogeneous-oxidation catalysts are normally poorly protected from self-destruction under operating conditions. Achieving design control over both activity and half-life is important not only in advancing the utility of oxidation catalysts, but also in minimizing hazards associated with their use and disposal. Iron(III)-TAML (tetraamido-macrocyclic ligand) oxidant catalysts rapidly activate H(2)O(2) for numerous significant processes, exhibiting high and differing…Orange II (Aldrich) was purified by passing it through a column filled with SMT Bulk-C18 (Separation Methods Technologies, Newark) with a water/methanol mixture (9:1) as eluent. - Methanocarba cycloakyl nucleoside analogues: United States Patent 7087589 Issued on August 2006.
The present invention provides novel nucleoside and nucleotide derivatives that are useful agonists or antagonists of P1 or P2 receptors. For example, the present invention provides a compound of formula A-M, wherein A is modified adenine or uracil and M is a constrained cycloalkyl group. The adenine or uracil is bonded to the constrained cycloakyl group…Purity of compounds was checked using a Hewlett-Packard 1090 HPLC apparatus equipped with an SMT OD-5-60 RP-C18 analytical column (250×4.6 mm; Separation Methods Technologies, Inc., Newark, Del.)… - Sulfonamidomethyl phosphonate inhibitors of I2-lactamase: United States Patent 7030103 Issued on April 2006.
The intention relates to bacterial antibiotic resistance and, in particular, to compositions and methods for overcoming bacterial antibiotic resistance. The invention provides novel β-lactamase inhibitors, which are structurally unrelated to the natural product and semi-synthetic β-lactamase inhibitors…The resultant crude product was more than 85% pure, but final purification was accomplished by preparative HPLC on an SMT C18 column using water/acetonitrile gradient… - Classiï¬cation of special octadecyl-bonded phases by the carotenoid test. Journal of Chrom A, January 2006
E. Lesellier, C. West, A. Tchapla
The SMT silica (C18/C1) (#68), obtained by horizontalpolymerization silanization method, seems not to avoid thesilanophilic interactions, judging by the low value of the carotene/zeaxanthin separation factor… - A rp-HPLC method for the assay of cefpirome and its application in drug-metal interaction studies. Pak J Pharm Sci. January 2006; 19(1):39-44.
Arayne MS1, Sultana N, Nawaz M.
An accurate, sensitive and least time consuming RP-HPLC method for the estimation of cefpirome in the presence of essential and trace metal has been developed and validated. Cefpirome was eluted from a B144A, OD-5-100, C(18) (150 x 4.6 mm) column at room temperature with a mobile phase consisting of MeOH:H2O (15:85, % v/v) at a flow rate of 1 ml/minute, while UV detection was performed at 265 nm. The detection limit of cefpirome was 10 ng. Drug metal interaction studies were carried out at 37 degrees C to monitor the complexation of drug with metal ions. These studies were beneficial to determine the drug in therapeutic concentrations inside human body as well as its complexation with metal cations… - Beta-Homolysine conjugates and their use as transport enhancer: European Patent EP1478405. Issued on June 2005.
Garcia-Echeverria Carlos, Novartis Ag, Novartis Pharma Gmbh
The present invention relates to a conjugate that comprises a) at least one compound to be delivered into or across a biological barrier, b) a delivery-enhancing transporter comprising at least 4 β-homolysine residues, especially between 4 and 25 β-homolysine residues, c) optionally a linker between the components a) and b), and d) optionally a labelling unit; to the salts of such conjugates; to a pharmaceutical composition…TFA from 1:49 to 1:0 and 3 min at 1:0; flow rate 2.0 mL/min, detection at 215 nm; SMT C18 column (250 x 4.6 mm; 5 µm, 100 A)… - Organic compounds having Anti-angiogenic activity: European Patent EP1478665 November 2004.
Carlos Garcia-Echeverria, Claire Lewis, Jeffery Robinson
he invention relates to the anti-angiogenic polypeptides and modified polypeptides derived from fibrinogen including oligomers of said polypeptides. Angiogenesis, the development of new blood vessels from an existing vascular bed, is a complex multistep process that involves the degradation of components of the extracellular matrix and then the migration, proliferation and differentiation of endothelial cells to form tubules and eventually new vessels…Gradient 8: linear gradient over 7 min of MeCN/0.09% TFA and H2O/0.1% TFA from 1:49 to 1:0 and 3 min at 1:0; flow rate 2.0 mL/min, detection at 215 nm; SMT C18 column, 5 µm, 250 x 4.6 mm… - Determination of Chromium(III) Picolinate Using High Performance Liquid Chromatography-Ultraviolet Spectrophotometry: Bull. Korean Chem. Soc. 2003, 24, 1517-1520.
Sung-Il Kim, Dong-Jin Woo, Myung-Hee Lee, Gun-Jo Woo, Dae-Kyung Kang, and Ki-Won Cha
Chromium(III) is very well known as an essential mineral.1,2 It is suggested as a cofactor in the maintenance of both normal lipid and carbohydrate metabolism by assisting the action of insulin on a cell membrane.3,4 According to the National Research Council, the daily recommended intake of chromium(III) is 50-200 μg.5 Several organic chromium(III) complexes have been reported to have significantly higher absorption and tissue incorporation…The octadesyl bonded column (total coverage B191A, octadesyl silica, 5 µm particle size, 4.6 × 250 mm I.D.) was purchased from Separation Methods Technologies… - Optical and Chromaticity Characteristics of Adsorbates of the Thorium Complex of Arsenazo I: Journal of Analytical Chemistry 2003, 58, 318-321.
V. M. Ivanov, N. I. Ershova, V. N. Figurovskaya
The immobilized reagent Arsenazo I was used for the direct determination of trace thorium by diffuse reflectance spectrometry and chromaticity measurements…silica gel C18 (SMT Bulk C18, Cat#: BOD-35-150, Ref#: 990226-B169A, Newark, DE) were used as adsorbents. - Structure−Activity Relationships at Human and Rat A2BAdenosine Receptors of Xanthine Derivatives
Substituted at the 1-, 3-, 7-, and 8-Positions
Soon-Ai Kim, Melissa A. Marshall, Neli Melman, Hak Sung Kim, Christa E. Müller, Joel Linden, and Kenneth A. Jacobson J. Med. Chem., 2002, 45 (11), pp 2131–2138 Publication Date (Web): April 25, 2002 (Article) DOI: 10.1021/jm0104318
Phenyl ring-substituted analogues of 49 were also tested: The electron-withdrawing 2,6-difluoro and 4-chloro groups on the 8- aromatic ring, in 53 and60, reduced affinity. … Where needed, determinations of purity were performed with a Hewlett- Packard 1090 high-performance liquid chromatography (HPLC) system using an SMT OD-5−60 C18 analytical column (250 mm × 4.6 mm, Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems… - Adenine Nucleotide Analogues Locked in a Northern Methanocarba Conformation: Enhanced Stability and Potency as P2Y1 Receptor Agonists R. Gnana Ravi, Hak Sung Kim, Jörg Servos, Herbert Zimmermann, Kyeong Lee, Savitri Maddileti, José L. Boyer, T. Kendall Harden, and Kenneth A. Jacobson J. Med. Chem., 2002, 45 (10), pp 2090–2100 Publication Date (Web): April 12, 2002 (Article) DOI: 10.1021/jm010538v
Purity of compounds was checked using a Hewlett-Packard 1090 HPLC apparatus equipped with an SMT OD-5-60 RP-C18 analytical column (250 mm × 4.6 mm; Separation Methods Technologies, Inc., Newark, DE) in two solvent systems. … (1'S,2'R,3'S,4'R,5'S)-4-(6-Amino-9H-purin-9-yl)-1-[(phenyl methoxy)methyl]bicyclo[3.1.0 ]hexane-2,3-(O-isopropyl-idene) (23)… - Methanocarba Modification of Uracil and Adenine Nucleotides: High Potency of Northern Ring Conformation at P2Y1, P2Y2, P2Y4, and P2Y11 but Not P2Y6 Receptors
Hak Sung Kim, R. Gnana Ravi, Victor E. Marquez, Savitri Maddileti, Anna-Karin Wihlborg, David Erlinge, Malin Malmsjö, José L. Boyer, T. Kendall Harden, and Kenneth A. Jacobson J. Med. Chem., 2002, 45 (1), pp 208–218
DOI: 10.1021/jm010369e Purity of all compounds in solvent 1 and compounds 5a, 6a, and 7a in solvent 2 was checked using an SMT OD-5-60 RP-C18 analytical column … Compounds 3a, 4a,8a, and 9a were checked in solvent 2 using a Phenomenex Luna 5μ C18(2) analytical column (250 × 4.6 mm)…Purity of all compounds in solvent 1 and compounds 5a, 6a, and 7a in solvent 2 was checked using an SMT OD-5-60 RP-C18 analytical column (250 × 4.6 mm; Separation Methods Technologies, Inc., Newark, DE) in two solvent systems. …(1'S,2'R,3'S,4'R,5'S)-4-(6-Amino-9H-purin-9-yl)-1-[phosphoryloxymethyl]bicyclo[3.1.0]hexane-2,3-diol (4a)… - Towards an understanding of biological role of colostrinin peptides: December 2001
Marian L. Kruzel, Maria Janusz, Jozef Lisowski, Robert V. Fischleigh, Jerzy A. Georgiades
The aim of this study was to elucidate the structure and possible function of colostrinin, also known as a proline rich polypeptide (PRP). The molecular weight of colostrinin was originally determined by gel filtration to be 17,200 daltons. In the presence of guanidinum chloride, however, the molecular weight was found to be about 6,000 daltons…High-Performance Liquid Chromatography (HPLC) HPLC analysis of colostrinin was carried out on a C18 column (pore size 300Å, particle size 3 micrometer, obtained from
Separation Methods Technologies Inc.), using Beckman System Gold 126… - Acyclic and Cyclopropyl Analogues of Adenosine Bisphosphate Antagonists of the P2Y1 Receptor: Structure−Activity Relationships and Receptor Docking
Hak Sung Kim, Dov Barak, T. Kendall Harden, José L. Boyer, and Kenneth A. Jacobson J. Med. Chem., 2001, 44 (19), pp 3092–3108 Publication Date (Web): August 22, 2001 (Article) DOI: 10.1021/jm010082h
The determinations of purity were performed with a Hewlett-Packard 1090 HPLC system using an SMT OD-5-60 C18 analytical column (250 mm × 4.6 mm, Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems. … After removal of volatile materials in vacuo, the residue was purified by column chromatography (ethyl acetate/methanol = 10:1) to give diol 77 (275 mg, 95%)… - Direct determination of phosphorilated intercellular anabolites of stavudine (d4T) by liquid chromatography/tandem mass spectrometry: RCM 2001, 15, 1401-1408.
Pruvost A, Becher F, Bardouille P, Guerrero C, Creminon C, Delfraissy JF, Goujard C, Grassi J, Benech H.
The objective was to develop and validate a routine assay for active intracellular anabolites of stavudine (d4T), a nucleoside reverse transcriptase inhibitor in human PBMC, applicable to pharmacokinetic studies and treatment monitoring. This was achieved using liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS)…Several types of columns were also tested both in normal diOH-YMC) and reverse phase YMC ODS, Kromasil C18, SMT C18). It was necessary to separate such polar compounds from each other and from the solvent front to allow efficient ionisation in the electrospray source. Finally, best results were obtained with DMH as ion-pairing reagent, formate as buffer, SMT C18 as the chromatographic column and acetonitrile as organic solvent… - Silica and other materials as supports in liquid chromatography, chromatographic tests and their importance for evaluating these supports, Part I: Chromatographia 2001, 53, S113-S131.
C. Stella, S. Rudaz, J. -L. Veuthey, A. Tchapla
Reversed-phase liquid chromatography (RP-HPLC) has become a powerful and widely employed technique in the separation and analysis of a great variety of compounds with different functionalities…This dense, two-dimensional cross-linked network over the chromatographic silica surface reduces the acid dissociation of surface silanols [24, 158 162]. This phase (SMT C18) can be compared both to monolayer densely bonded silicas and specific bonded mixed silicas… - Determination of Aluminum and Beryllium by Diffuse Reflectance Spectrometry with the Use of Chromaticity Functions: Journal of Analytical Chemistry 2001, 56, 1089-1094.
V. M. Ivanov, N. I. Ershova
Immobilized Eriochrome Cyanine R was used for the direct determination of trace aluminum and beryllium by diffuse reflectance spectrometry. Anion exchanger AV-17, silica gel Silochrom C-120, Chromaton N-Super, octadecyl silica gel, and cellulose were examined as supports. Optimal sorption conditions were found. The dependence of chromaticity…and silica gel C18 (SMT Bulk C18, Cat. no. BOD-35-150, Ref. no. 990 226-B169A, Newark, Germany)… - Structure-Activity Relationships of Pyridoxal Phosphate Derivatives as Potent and Selective Antagonists of P2Xâ‚ Receptors
Yong-Chul Kim, Sean G. Brown, T. Kendall Harden, José L. Boyer, George Dubyak, Brian F. King, Geoffrey Burnstock, and Kenneth A. Jacobson J. Med. Chem., 2001, 44 (3), pp 340–349 Publication Date (Web): December 19, 2000 (Article) DOI: 10.1021/jm9904203
The determinations of purity were performed with a Hewlett-Packard 1090 HPLC system using an OD-5- 60 C18 analytical column (250 mm × 4.6 mm; Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems… - Methanocarba Analogues of Purine Nucleosides as Potent and Selective Adenosine Receptor Agonists
Kenneth A. Jacobson, Xiao-duo Ji, An-Hu Li, Neli Melman, Maqbool A. Siddiqui, Kye-Jung Shin, Victor E. Marquez, and R. Gnana Ravi J. Med. Chem., 2000, 43 (11), pp 2196–2203 Publication Date (Web): May 16, 2000 (Article)
DOI: 10.1021/jm9905965 The determination of purity was performed with a Hewlett-Packard 1090 HPLC system using an SMT OD-5-60 C18 analytical column (250 mm × 4.6 mm, Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems. … Solvent was removed under vacuum, and the residue obtained was purified by flash column chromatography using 7/3 chloroform/methanol to furnish 3.0 mg (47%) of the product, mp. 85 °C… - Anilide Derivatives of an 8-Phenylxanthine Carboxylic Congener Are Highly Potent and Selective Antagonists at Human A2B Adenosine Receptors
Yong-Chul Kim, Xiao-duo Ji, Neli Melman, Joel Linden, and Kenneth A. Jacobson J. Med. Chem., 2000, 43 (6), pp 1165–1172 Publication Date (Web): February 26, 2000 (Article) DOI: 10.1021/jm990421v
Where needed, evaluation of purity was done on a Hewlett-Packard 1090 HPLC system using an OD-5-60 C18 analytical column (150 mm × 4.6 mm; Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems, at a flow rate of 1 mL/min… - Synthesis, Biological Activity, and Molecular Modeling of Ribose-Modified Deoxyadenosine Bisphosphate Analogues as P2Yâ‚ Receptor Ligands
Erathodiyil Nandanan, Soo-Yeon Jang, Stefano Moro, Hea Ok Kim, Maqbool A. Siddiqui, Pamela Russ, Victor E. Marquez, Roger Busson, Piet Herdewijn, T. Kendall Harden, José L. Boyer, and Kenneth A. Jacobson J. Med. Chem., 2000, 43 (5), pp 829–842 Publication Date (Web): February 23, 2000 (Article)
Purity of compounds was checked using a Hewlett-Packard 1090 HPLC apparatus equipped with an SMT OD-5-60 RP-C18 analytical column (250 × 4.6 mm; Separation Methods Technologies, Inc., Newark, DE) in two solvent systems…The reaction was monitored by analytical HPLC (eluting with a gradient consisting of buffer:CH3CN in the ratio 95:5 to 40:60, in which the buffer was 0.1 M triethylammonium acetate (TEAA); elution time was 20 min; flow rate was 1 mL/min; column was SMT OD-5- 60 RP-C18; detector was by UV in the Emax range of 260−300 nm)… - Acyclic Analogues of Deoxyadenosine 3‘,5‘-Bisphosphates as P2Y1 Receptor Antagonists
Yong-Chul Kim, Carola Gallo-Rodriguez, Soo-Yeon Jang, Erathodiyil Nandanan, Mary Adams, T. Kendall Harden, José L. Boyer, and Kenneth A. Jacobson J. Med. Chem., 2000, 43 (4), pp 746–755 Publication Date (Web): February 09, 2000 (Article) DOI: 10.1021/jm9905211
The determinations of purity were performed with a Hewlett-Packard 1090 HPLC system using an SMT OD- 5-60 C18 analytical column (250 mm × 4.6 mm, Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems… - Measurement of Food Flavonoids by High-Performance Liquid Chromatography: A Review
Howard M. Merken and Gary R. Beecher J. Agric. Food Chem., 2000, 48 (3), pp 577–599 Publication Date (Web): February 29, 2000 (Review) DOI: 10.1021/jf990872o
After HPLC, samples can be further purified for mass spectrometry (MS) using column chromatography, as was done for Zambian munkoyo beverage (Zulu et al., 1994). … Zorbax Rx-C18 and SMT OD-5-100 columns also worked for system 1… - Arsenic Speciation in Human Organs following Fatal Arsenic Trioxide Poisoning – A Case Report: Clinical Chemistry 1999, 45, 301-306.
Benramdane L1, Accominotti M, Fanton L, Malicier D, Vallon JJ.
The aim of this investigation was to study the distribution of arsenic species in human organs following fatal acute intoxication by arsenic trioxide. The collected autopsy samples of most organs were ground and dried, and the total arsenic was measured by electrothermal atomic absorption spectrometry (ETAAS). The arsenic species–inorganic arsenic, in the form of arsenite [As(III)] and arsenate [As(V)], and its metabolites [monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA)]–were quantified by ETAAS after extraction with methanol/water (1:1, by volume) and separation by HPLC…Separation was performed in ion-pair mode on a reversed-phase silica-based octadecyl (C18) column resistant at pH 2–12 pH (SMT OD-5-100, 250 4.6 mm i.d.)… - Determination of zolpidem in serum microsamples by high-performance liquid chromatography and its application to pharmacokinetics in rats: November 1999
Qiao Wang; Lei Sun; Chyan E. Lau
A single-solvent extraction step high-performance liquid chromatographic method is described for quantitating zolpidem in rat serum microsamples (50 microl). The separation used a 2.1 mm I.D. reversed-phase OD-5-100 C18 column, 5 microm particle size with an isocratic mobile phase consisting of methanol-acetonitrile-26 mM sodium acetate buffer (adjusted to pH 2.0 with 40% phosphoric acid) containing… - Chiral Resolution and Stereospecificity of 6-Phenyl-4-phenylethynyl- 1,4-dihydropyridines as Selective A3Adenosine Receptor Antagonists
Ji-long Jiang, An-Hu Li, Soo-Yeon Jang, Louis Chang, Neli Melman, Stefano Moro, Xiao-duo Ji, Emil B. Lobkovsky, Jon C. Clardy, and Kenneth A. Jacobson J. Med. Chem., 1999, 42 (16), pp 3055–3065 Publication Date (Web): July 22, 1999 (Article)
The essential modifications leading to A3 selectivity and preventing binding to L-type calcium channels and other sites are a phenyl ring at the 6-position and at the 4-position either a styryl or a phenylethynyl group (e.g. 1 and 2, Figure 1). … Purity of diastereomers 9−12 and enantiomers of MRS 1191, compounds 21 (4S)and 23 (4R), was checked by HPLC using SMT OD-5-60 RP C18 (250 × 4.6 mm) as an analytical column in two solvent systems…Purity of diastereomers 9−12 and enantiomers of MRS 1191, compounds 21 (4S) and 23 (4R), was checked by HPLC using SMT OD-5-60 RP C18 (250 × 4.6 mm) as an analytical column in two solvent systems. Purity of diastereomers 9−12 and enantiomers of MRS 1191, compounds 21 (4S)and 23 (4R), was checked by HPLC using SMT OD-5-60 RP C18 (250 × 4.6 mm) as an analytical column in two solvent systems… - Structure-Activity Relationships of Bisphosphate Nucleotide Derivatives as P2Yâ‚ Receptor Antagonists and Partial Agonists
Erathodiyil Nandanan, Emidio Camaioni, Soo-Yeon Jang, Yong-Chul Kim, Gloria Cristalli, Piet Herdewijn, John A. Secrist, III, Kamal N. Tiwari, Arvind Mohanram, T. Kendall Harden, José L. Boyer, and Kenneth A. Jacobson
Publication Date (Web): April 21, 1999 (Article) DOI: 10.1021/jm980657j Purity of compounds was checked by a Hewlett-Packard 1090 HPLC apparatus using SMT OD-5-60 RP-C18 (250 × 4.6 mm; Separation Methods Technologies, Inc., Newark, DE) as an analytical column in two solvent systems. … The reaction was monitored by analytical HPLC (eluting with gradient from buffer, CH3CN = 95:5 to 40:60; buffer, 0.1 M triethylammonium acetate (TEAA); elution time, 20 min; flow rate, 1 mL/min; column, SMT OD-5-60 RP-C18; detector, UV, Emax 260-300 nm)… - Derivatives of the Triazoloquinazoline Adenosine Antagonist (CGS 15943) Having High Potency at the Human A2B and A3 Receptor Subtypes
Yong-Chul Kim, Maarten de Zwart, Louis Chang, Stefano Moro, Jacobien K. von Frijtag Drabbe Künzel, Neli Melman, Ad P. IJzerman, and Kenneth A. Jacobson J. Med. Chem., 1998, 41 (15), pp 2835–2845 Publication Date (Web): June 18, 1998 (Article) DOI: 10.1021/jm980094b
Where needed, evaluation of purity was done on a Hewlett-Packard 1090 HPLC system using an OD-5- 60 C18 analytical column (150 mm × 4.6 mm; Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems… - Deoxyadenosine Bisphosphate Derivatives as Potent Antagonists at P2Yâ‚ Receptors
Emidio Camaioni, José L. Boyer, Arvind Mohanram, T. Kendall Harden, and Kenneth A. Jacobson
J. Med. Chem., 1998, 41 (2), pp 183–190 Publication Date (Web): January 15, 1998 (Article) DOI: 10.1021/jm970433l Purity of compounds was checked by a Hewlett-Packard 1090 HPLC apparatus using SMT OD-5-60 RP-C18 (250 × 4.6 mm; Separation Methods Technologies, Inc., Newark, DE) as an analytical column in two-solvent systems. … The reaction was monitored by analytical HPLC (eluting with gradient from buffer:CH3CN = 95:5 to 40:60; buffer, 0.1 M triethylammonium acetate (TEAA); elution time, 20 min; flow rate, 1 mL/min; column, SMT OD-5-60 RP-C18… - A Pyridoxine Cyclic Phosphate and Its 6-Azoaryl Derivative Selectively Potentiate and Antagonize Activation of P2Xâ‚ Receptors
Kenneth A. Jacobson, Yong-Chul Kim, Scott S. Wildman, Arvind Mohanram, T. Kendall Harden, José L. Boyer, Brian F. King, and Geoffrey Burnstock J. Med. Chem., 1998, 41 (13), pp 2201–2206 Publication Date (Web): June 03, 1998 (Expedited Article)
DOI: 10.1021/jm980183o Determination of purity were performed with a Hewlett-Packard 1090 HPLC system using an OD-5-60 C18 analytical column (250 mm × 4.6 mm, Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems… - Derivatives of the Triazoloquinazoline Adenosine Antagonist (CGS15943) Are Selective for the Human A3 Receptor Subtype
Yong-Chul Kim, Xiao-duo Ji, and Kenneth A. Jacobson
J. Med. Chem., 1996, 39 (21), pp 4142–4148 Publication Date (Web): October 11, 1996 (Expedited Article) DOI: 10.1021/jm960482i Where needed evaluation of purity was done on a Hewlett-Packard 1090 HPLC system using OD-5-60 C18 analytical column (150 mm × 4.6 mm, Separation Methods Technologies, Inc., Newark, DE) in two different linear gradient solvent systems…
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