USP L Column Listing

The following chart has been provided as a guide to selecting HPLC columns which meet the specifications set forth in the United States Pharmacopeia [USP], which provides guidelines for the separation of related compounds.
 
It is important to note that, in many cases, USP column specifications are so broad that several (or many) column types actually meet the basic specications. For example, L1 specification calls for a column consisting of silica packing material, 5 or 10μm in diameter, bonded with octadecyl (C18) silane. However, a limited number of available C18 columns will actually perform the desired separation.
 
SMT offers a variety of columns for each category. These columns are representative of the wide range of selectivities available for each bonded phase. The packing materials vary in particle size, pore size, surface area, carbon load, hydrophobicity, bonded phase coverage or density, and other characteristics. Refer to the catalog for description and characteristics of a specific column of interest.
 

United States Pharmacopeia [USP]
ColumnDescriptionColumn(s)
L1Octadecylsilane chemically bonded to porous or non-porous silica or ceramic microparticles, 1.5 to 10μm in diameterOD
ODL
Elite C18
Symplex C18
Supreme C18
AquSep C18
L2Octadecylsilane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50μm in diameterBOD Bulk Packings
BODL Bulk Packings
L3Porous silica particles, 1.5 to 10μm in diameterS
L4Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50μm in diameterBS Bulk Packings
L7Octylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10μm in diameterO
OL
Elite C8
L8Aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10μm in diameterAP (NH2)
L9Porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10μm in diameterSCX
L10Nitrile groups chemically bonded to porous silica particles, 1.5 to 10μm in diameterCP (CN)
L11Phenyl groups chemically bonded to porous silica particles, 1.5 to 10μm in diameterPhen1
Phen2
L12A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50μm in diameterBSAX Bulk Packings
L13Trimethylsilane chemically bonded to porous silica particles, 3 to 10μm in diameterMEB1 C1
L14Silica gel having a chemicallly bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10μm in diameterSAX
L15Hexylsilane chemically bonded to totally porous silica particles, 3 to 10μm in diameterHex C6
L16Dimethylsilane chemically bonded to porous silica particles, 5 to 10μm in diameterMEB2-DM C2
L18Amino and cyano groups chemically bonded to porous silica particles, 3 to 10μm in diameterAP-CP
L20Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10μm in diameterDIOL2
L26Butylsilane chemically bonded to totally porous silica particles, 1.5 to 10μm in diameterMEB4 C4
L27Porous silica particles, 30 to 50μm in diameterBS Bulk Packings
L30Ethyl silane chemically bonded to 3-10µm porous silicaMEB2 C2
L40Cellulose tris-3,5-dimethylphenylcarbamate bonded on porous silica, 5-20µmChiralSep1
L41Immobilised α1-acid glycoprotein on spherical silica particles, 5μm in diameterChiralSepAGP
L42Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5μm in diameterODO
L43Pentafluorophenyl groups chemically bonded to silica particles 5 to 10µm in diameterC6F5
L44Spherical silica substrate that has been bonded with a cationic exchanger, sulphonic L44 acid functionality in addition to a conventional reversed phase C8 functionalitySCX-O
L52Strong Cation Exchange resin made of porous silica with sulfopropyl groups, 5 to 10µm in diameterSCX
L56Propyl silane chemically bonded to totally porous silica particles, 3 to 10μm in diameterPro
L57Chiral recognition protein, ovomucoid, chemically bonded to silica particles, about 5 µm in diameter, with pore size of 120ÅCIB Ovomucoid
L59Packing having the capacity to separate proteins by molecular weight over the range of 10 to 500 kDa. It is spherical (10 μm), silica-based, and processed to provide hydrophilic characteristics and pH stability SMTgel Diol
L60Spherical porous silica gel, 3 or 5 µm diameter, the surface of which has been covalently modified with alkyl amide groupsAquSep2
L62C30 silane bonded phase on a fully porous spherical silica, 5-10µm in diameterTA
L63Glycopeptide teicoplanin linked through multiple covalent bonds to spherical silicaCIB TE
L70Cellulose tris(phenyl carbamate) coated on 5-10µm porous silicaChiralSep2
L80Cellulose tris(4-methylbenzoate)-coated, porous, spherical, silica particles, 5 to 20 μm in diameterChiralSep3
L85A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and weak cation-exchange (carboxyl groups) functional groups chemically bonded to porous or non-porous particles, 1.0 to 50 µm in diameterWCX-O
L93Cellulose tris(3,5-dimethylpheylcarbamate) reversed phase chiral stationary phase coated on 3 or 5 µm silica gel particlesChiralSep1
L107Cellulose tris(4-methylbenzoate)-coated porous spherical particles, 3 to 5 µm in diameterChiralSep3
UnclassifiedDodecyl silane bonded on 5-10µm porous silicaDD
UnclassifiedProprietary strong cation exchange groups on 5-50µm Porous silicaMetalSep