Referenced Articles


Sequencing Grade Tandem Mass Spectrometry for Top-Down Proteomics Using Hybrid Electron Capture Dissociation Methods in a Benchtop Orbitrap Mass Spectrometer – SMT REFERENCED ARTICLE

Shaw JB, Malhan N, Vasil’ev YV, Lopez NI, Makarov A, Beckman JS, Voinov VG
Analytical Chemistry, 2018

American Journal of Microbiological Research, Separation Methods TechnologiesCompared to traditional collision induced dissociation methods, electron capture dissociation (ECD) provides more comprehensive characterization of large peptides and proteins as well as preserves labile post-translational modifications. However, ECD experiments are generally restricted to the high magnetic fields of FTICR-MS that enable the reaction of large polycations and electrons. Here, we demonstrate the use of an electromagnetostatic ECD cell to perform ECD and hybrid ECD methods utilizing 193 nm photons (ECuvPD) or collisional activation (EChcD) in a benchtop quadrupole-Orbitrap mass spectrometer…and were packed in-house with 3 µm 300 AÌŠ C2 [BMEB2-5-300] reversed phase resin (Separation Methods Technologies, INC., Newark, DE). mAb IdeS digest (0.1 μg) was loaded onto the trap column and washed for 10 min at 5 µL/min with 1% mobile phase B…READ MORE HERE


Extraction of Phytochemicals from Eucalyptus Spp. & Withania Somnifera and Their Biological Testing – SMT REFERENCED ARTICLE

Praveen Kumar Gupta, Priyanka V, Lingayya Hiremath, S Narendra Kumar, Ajeet Kumar Srivastava
American Journal of Microbiological Research, 2018

American Journal of Microbiological Research, Separation Methods TechnologiesDisease incidence and prevalence is increasing in developing countries leading to high mortality and morbidity rates. Since most of the developing countries rely on traditional plant based medicine, there is huge demand for identifying the bioactive compounds from plant origin with medicinal properties owing to their safety, availability and reduced side effects. This project focuses on extraction of two different phytochemicals from Eucalyptus Spp and Withania Somnifera…HPLC analysis was carried out on a LC-20AT system (Shimadzu, Japan) using a reverse-phase C18 column [OD-5-100/25] (4.6 mm x 250 mm; Separation Methods Technologies)…READ MORE HERE


Informed-Proteomics: open-source software package for top-down proteomics – SMT REFERENCED ARTICLE

Jungkap Park, Paul D Piehowski, Christopher Wilkins, Mowei Zhou, Joshua Mendoza, Grant M Fujimoto, Bryson C Gibbons, Jared B Shaw, Yufeng Shen, Anil K Shukla, Ronald J Moore, Tao Liu, Vladislav A Petyuk, Nikola Tolić, Ljiljana Paša-Tolić, Richard D Smith, Samuel H Payne & Sangtae Kim
Nature Methods, Volume 14

Nature Methods, Volume 14 Separation Methods TechnologiesTop-down proteomics, the analysis of intact proteins in their endogenous form, preserves valuable information about post-translation modifications, isoforms and proteolytic processing. The quality of top-down liquid chromatography–tandem MSMS (LCLC-MSMS/MSMS) data sets is rapidly increasing on account of advances in instrumentation and sample-processing protocols. However, top-down mass spectra are substantially more complex than conventional bottom-up data…The analytical column was prepared in house by slurry packing 3 µm diameter C2 [BMEB C2] stationary phase (Separation Methods Technology, Newark, Delaware) into a 50 cm length of 360 µm o.d. × 100 µm i.d. fused silica capillary column…READ MORE HERE


High-Resolution Ultrahigh-Pressure Long Column Reversed-Phase Liquid Chromatography for Top-down Proteomics – SMT REFERENCED ARTICLE

Yufeng Shen, Nikola Tolić, Paul D. Piehowski, Anil K. Shukla, Sangtae Kim, Rui Zhao, Yi Qu, Errol Robinson, Richard D. Smith
Journal of Chromatography A

Journal-of-Chromatography-A Separation Methods TechnologiesSeparation of proteoforms for global intact protein analysis (i.e. top-down proteomics) has lagged well behind what is achievable for peptides in traditional bottom-up proteomic approach and is becoming a true bottle neck for top-down proteomics. Herein, we report use of long (≥1 M) columns containing short alkyl (C1-C4) bonded phases to achieve high-resolution RPLC for separation of proteoforms…The packing…silica particles, were from…Separation Methods Technologies (Newark, DE) [3µm, 300Å C1 porous silica (BMEB1-3-300) and 3µm, 300Å C2 porous silica (BMEB2-3-300)]…C1 RPLC has the greatest potential in terms of elution of hydrophobic and larger proteoforms; accordingly, we observed dense peak arrays in the C1 RPLC late elution zone…READ MORE HERE


Novel Biosensor of Membrane Protein Proximity Based on Fluorogen Activated Proteins – SMT REFERENCED ARTICLE

V. Vasilev, Kalin; Gallo, Eugenio; Shank, Nathaniel; W. Jarvik, Jonathan
Combinatorial Chemistry & High Throughput Screening

CCTHSThe biosensor takes advantage of recently developed fluorogen-activating proteins (FAPs) that display fluorescence only when bound to otherwise-nonfluorescent fluorogen molecules…The bright blue product was purified…with a column packed with Bulk C18 (Separation Methods Technologies) [BOD-35-150] using a linear gradient of 0.1% trifluoroacetic acid (TFA) in H2O and acetonitrile (ACN) (0% to 40% ACN over 40…READ MORE HERE


Interactions of Haptoglobin with Monomeric Globin Species: Insights from Molecular Modeling and Native Electrospray Ionization Mass Spectrometry – SMT REFERENCED ARTICLE

Ololade Fatunmbi, Rinat R. Abzalimov, Sergey N. Savinov, Anne Gershenson & Igor A. Kaltashov
Biochemistry – ACS
Biochemistry - ACSHaptoglobin (Hp) binds free hemoglobin (Hb) dimers to prevent negative consequences of Hb circulation in the extracellular environment. Although both monomeric Hb and myoglobin (Mb) species also present potential risks, their interactions with Hp have not been extensively studied. Mb is homologous to both the α- and β-chains of Hb and shares many conserved Hb/Hp interface residues, yet whether Hp binds Mb remains unclear…Hb α- and β-chains were separated via reversed phase high-performance liquid chromatography…using a 4.6 mm × 150 mm C8 [O-5-100/15] analytical column with a 5 µm pore size (Separation Methods Technologies, Newark, DE)… READ MORE HERE


Kinetic and Mechanistic Studies of the Reactivity of Iron(IV) TAMLs Toward Organic Sulfides in Water: Resolving a Fast Catalysis Versus Slower Single-Turnover Reactivity Dilemma – SMT REFERENCED ARTICLE

Deboshri Banerjee, Alexander D. Ryabov & Terrence J. Collins
Journal of Coordination Chemistry

TAML complex is oxidized by H2O2 or tBuOOH in water at pH < 10 into the corresponding iron(IV) μ-oxo-bridged dimer 2, which oxidizes readily ring-substituted thioanisoles p-XC6H4SMe (X=H, MeO, Me, Cl, CN) into the corresponding sulfoxides with regeneration of 1. The oxidation studied under pseudo-first-order conditions using the stopped-flow technique by monitoring the fading of the 420-nm band of 2 follows hyperbolic kinetics according to the rate law kobs = ab[p-XC6H4SMe]/(1 + b[p-XC6H4SMe]) at pH 8 and 25 °C…. Orange II was purified by passing through a column filled with SMT Bulk-C18 (Separation Methods Technologies Inc., Newark) with water–methanol mixture (9 : 1) as an eluent… READ MORE HERE


Fe(III)–TAML-Catalyzed Green Oxidative Degradation of the Azo Dye Orange II by H2O2 and Organic Peroxides: Products, Toxicity, Kinetics, and Mechanisms — SMT REFERENCED ARTICLE

Green ChemistryNaima Chahbane, Delia-Laura Popescu, Douglas A. Mitchell, Arani Chanda, Dieter Lenoir, Alexander D. Ryabov, Karl-Werner Schramma and Terrence J. Collins
Green Chemistry

Oxidation of Orange II ([4-[(2-hydroxynaphtyl)azo]benzenesulfonic acid], sodium salt) by hydrogen peroxide catalyzed by iron(III) complexed to tetra amido macrocyclic ligands (FeIII–TAML activators) in aqueous solutions at pH 9–11 leads to CO2, CO, phthalic acid and smaller aliphatic carboxylic acids as major mineralization products. The products are non-toxic according to the Daphnia magna test. Several organic intermediates have been identified by HPLC and GC-MS that allowed the detailed description of Orange II degradation … 26 Ozone degrades practically all dyes, reacting rapidly with both C–N and N N bonds … for the oxidative degradation of Orange II by H2O2 and organic peroxides … For kinetic investigations, Orange II was purified by passing through a column filled with SMT Bulk-C18 (Separation Methods Technologies, Inc.)… READ MORE HERE


AMERICAN LABORATORY: Advances in Separation Science Technology and Applications at Pittcon 2015

AmericanLaboratory-1Self-assembly of a monolayer (SAM) stationary phase bonding is a novel concept developed by Separation Methods Technologies (SMT, Newark, Del.) for giving high surface coverage of silica bonding. Generally, high coverage improves stability and often reduces tailing. SMT claims coverage of 99.99% of the silanols compared to about 50% for conventional bonding, before end-capping. SAM bonding is especially useful in prep since the columns are so robust and peaks shapes are ideal.

By: Robert L. Stevenson, Ph.D.
Original Article


Development and Validation of HPLC Method for Simultaneous Estimation of Ketorolac Tromethamine and Ofloxacin in Bulk and Ophthalmic Formulation — SMT REFERENCED ARTICLE

Tambe Vrushali Sachin, Dr. Deodhar Meenakshi, Dr. Prakya Vijayalakshmi
Indo American Journal of Pharmaceutical Research, 2015

A novel validated HPLC method was developed for simultaneous determination of Ofloxacin and Ketorolac Tromethamine from bulk and eye drop formulation. This method is simple, precise, accurate and utilizes reverse phase SMT SAM-C18 column (4.6 × 250 mm) with a ternary mobile phase with pH adjustment to 2.7 with formic acid, at a flow rate of 1ml/min. Both drugs were qunatitated at 300 nm. The retention time for OFO and KTM was found to be 2.33 and 10.33 min respectively…