Shaw JB, Malhan N, Vasil’ev YV, Lopez NI, Makarov A, Beckman JS, Voinov VG
Analytical Chemistry, 2018
Compared to traditional collision induced dissociation methods, electron capture dissociation (ECD) provides more comprehensive characterization of large peptides and proteins as well as preserves labile post-translational modifications. However, ECD experiments are generally restricted to the high magnetic fields of FTICR-MS that enable the reaction of large polycations and electrons. Here, we demonstrate the use of an electromagnetostatic ECD cell to perform ECD and hybrid ECD methods utilizing 193 nm photons (ECuvPD) or collisional activation (EChcD) in a benchtop quadrupole-Orbitrap mass spectrometer…and were packed in-house with 3 µm 300 AÌŠ C2 [BMEB2-5-300] reversed phase resin (Separation Methods Technologies, INC., Newark, DE). mAb IdeS digest (0.1 μg) was loaded onto the trap column and washed for 10 min at 5 µL/min with 1% mobile phase B…READ MORE HERE
Disease incidence and prevalence is increasing in developing countries leading to high mortality and morbidity rates. Since most of the developing countries rely on traditional plant based medicine, there is huge demand for identifying the bioactive compounds from plant origin with medicinal properties owing to their safety, availability and reduced side effects. This project focuses on extraction of two different phytochemicals from Eucalyptus Spp and Withania Somnifera…HPLC analysis was carried out on a LC-20AT system (Shimadzu, Japan) using a reverse-phase C18 column [OD-5-100/25] (4.6 mm x 250 mm; Separation Methods Technologies)…
Top-down proteomics, the analysis of intact proteins in their endogenous form, preserves valuable information about post-translation modifications, isoforms and proteolytic processing. The quality of top-down liquid chromatography–tandem MSMS (LCLC-MSMS/MSMS) data sets is rapidly increasing on account of advances in instrumentation and sample-processing protocols. However, top-down mass spectra are substantially more complex than conventional bottom-up data…The analytical column was prepared in house by slurry packing 3 µm diameter C2 [BMEB C2] stationary phase (Separation Methods Technology, Newark, Delaware) into a 50 cm length of 360 µm o.d. × 100 µm i.d. fused silica capillary column…
Separation of proteoforms for global intact protein analysis (i.e. top-down proteomics) has lagged well behind what is achievable for peptides in traditional bottom-up proteomic approach and is becoming a true bottle neck for top-down proteomics. Herein, we report use of long (≥1 M) columns containing short alkyl (C1-C4) bonded phases to achieve high-resolution RPLC for separation of proteoforms…The packing…silica particles, were from…Separation Methods Technologies (Newark, DE) [3µm, 300Å C1 porous silica (BMEB1-3-300) and 3µm, 300Å C2 porous silica (BMEB2-3-300)]…C1 RPLC has the greatest potential in terms of elution of hydrophobic and larger proteoforms; accordingly, we observed dense peak arrays in the C1 RPLC late elution zone…
The biosensor takes advantage of recently developed fluorogen-activating proteins (FAPs) that display fluorescence only when bound to otherwise-nonfluorescent fluorogen molecules…The bright blue product was purified…with a column packed with Bulk C18 (Separation Methods Technologies) [BOD-35-150] using a linear gradient of 0.1% trifluoroacetic acid (TFA) in H2O and acetonitrile (ACN) (0% to 40% ACN over 40…
Haptoglobin (Hp) binds free hemoglobin (Hb) dimers to prevent negative consequences of Hb circulation in the extracellular environment. Although both monomeric Hb and myoglobin (Mb) species also present potential risks, their interactions with Hp have not been extensively studied. Mb is homologous to both the α- and β-chains of Hb and shares many conserved Hb/Hp interface residues, yet whether Hp binds Mb remains unclear…Hb α- and β-chains were separated via reversed phase high-performance liquid chromatography…using a 4.6 mm × 150 mm C8 [O-5-100/15] analytical column with a 5 µm pore size (Separation Methods Technologies, Newark, DE)… 
TAML complex is oxidized by H2O2 or tBuOOH in water at pH < 10 into the corresponding iron(IV) μ-oxo-bridged dimer 2, which oxidizes readily ring-substituted thioanisoles p-XC6H4SMe (X=H, MeO, Me, Cl, CN) into the corresponding sulfoxides with regeneration of 1. The oxidation studied under pseudo-first-order conditions using the stopped-flow technique by monitoring the fading of the 420-nm band of 2 follows hyperbolic kinetics according to the rate law kobs = ab[p-XC6H4SMe]/(1 + b[p-XC6H4SMe]) at pH 8 and 25 °C…. Orange II was purified by passing through a column filled with SMT Bulk-C18 (Separation Methods Technologies Inc., Newark) with water–methanol mixture (9 : 1) as an eluent… 
Naima Chahbane, Delia-Laura Popescu, Douglas A. Mitchell, Arani Chanda, Dieter Lenoir, Alexander D. Ryabov, Karl-Werner Schramma and Terrence J. Collins
Self-assembly of a monolayer (SAM) stationary phase bonding is a novel concept developed by Separation Methods Technologies (SMT, Newark, Del.) for giving high surface coverage of silica bonding. Generally, high coverage improves stability and often reduces tailing. SMT claims coverage of 99.99% of the silanols compared to about 50% for conventional bonding, before end-capping. SAM bonding is especially useful in prep since the columns are so robust and peaks shapes are ideal.